Investigation of Optical Properties of Tissues in vivo for the purposes of PDT Dosimetry

Goals

• Recover concentration of PDT photosensitizer in tissues
• Study diffuse propagation of the light in tissues using multispectral spectrometer

Hardware and Data Acquisition

• System integration of a custom multispectral imaging spectrometer
• Custom fiberoptics probes for light delivery and signal registartion
• Custom LabVEIW software suite for system control and data acquisition

Light Propagation Modeling

• Propagation of laser light in tssues was modeled using diffusion approximation
• Simulated annealing - based data analysis allowed reconstruction of tissue absorption and scattering coefficients

Results

• For the first time, localized concentration of photosensitizer drug in internal organs was optically measured in vivo  (canine)
• Oxygen saturation for individual organs was estimated
• PDT dosimetry was improved as a result